Flow Gradient Separation of a Cold Mix on Gemini C18

LC Conditions

Column

Gemini 5 µm C18 110 Å, LC Column 150 x 4.6 mm, Ea

Brand

Gemini

Part No

00F-4435-E0

Phase Name

C18

Elution Type

Gradient

Mobile Phase

A: 10 mM Phosphate Buffer, pH 5.0

B: Methanol

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

60

40

2

3.9

60

40

3

3.91

60

40

4

10

60

40

Flow Rate

0.80 mL/min

Temperature

22°C

Detection

UV-Vis Abs.-Diode Array (PDA) @ (22°C)

Detection Info

Sample Notes

Flow gradient separation of four analytes in cold remedy on Gemini C18: Phenylephrine, Acetaminophen, Chlorpheniramine and Dextromethorphan and impurity of Maleic acid