Tryptic Map of Bovine Cytochrome C - Jupiter Proteo

LC Conditions

Column

Jupiter 4 µm Proteo 90 Å, LC Column 250 x 4.6 mm, Ea

Brand

Jupiter

Part No

00G-4396-E0

Phase Name

Proteo

Elution Type

Gradient

Mobile Phase

A: Water with 1.25mM TFA

B: Acetonitrile with 1mM TFA

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

95

5

2

5

95

5

3

60

60

40

Flow Rate

1.00 mL/min

Temperature

22°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info

Sample Preparation Details

Sample Pretreatment

asdrtsdrn adrtnr sftynstynszdty fgymzdxtyzdtm dtd

Load

Load Sample

Evaporation

Evaporate to dryness