PEGylated Ribonuclease A on Jupiter® 300 C18 3µm, reaction at different time-points

LC Conditions

Column

Jupiter 3 µm C18 300 Å, LC Column 150 x 4.6 mm, Ea

Brand

Jupiter

Part No

00F-4263-E0

Phase Name

C18

Elution Type

Gradient

Mobile Phase

A: 0.1% TFA, 2% ACN in Water

B: 90% ACN, 0.085% TFA in Water

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

80

20

2

25

35

65

Flow Rate

1.00 mL/min

Temperature

45°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (25°C)

Detection Info

Sample Notes

Separation of N-Terminus PEGylated Rebonuclease A with 20kDa Methoxy PEG aldehyde (PEG1) on Jupiter 300 3µm C18.