Loading Study: 16 mg at Low pH on Luna 5 µm C18(2) 50x21.2 mm Axia Packed

LC Conditions

Column

Luna 5 µm C18(2) 100 Å, LC Column 50 x 21.2 mm, AXIA Packed, Ea

Brand

Luna

Part No

00B-4252-P0-AX

Phase Name

C18(2)

Elution Type

Gradient

Mobile Phase

A: 0.5% TFA in Water, pH 2.0

B: 0.5% TFA in Acetonitrile

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

95

5

2

4

5

95

Flow Rate

30.00 mL/min

Temperature

22°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info

Sample Notes

SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard PREP Cartridge, C18 15 x 21.2mm ID, Ea Part No.: AJ0-7839 - Holder Part No.: AJ0-8223