Loading Study: 60 mg at High pH on Gemini 5 µm C18 50x21.2 mm Axia Packed

LC Conditions

Column

Gemini 5 µm C18 110 Å, LC Column 50 x 21.2 mm, AXIA Packed, Ea

Brand

Gemini

Part No

00B-4435-P0-AX

Phase Name

C18

Elution Type

Gradient

Mobile Phase

A: 50 mM Ammonium Bicarbonate, pH 10.0

B: Acetonitrile

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

95

5

2

5

5

95

Flow Rate

30.00 mL/min

Temperature

22°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info

Sample Notes

SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard PREP Cartridge, Gemini C18 15 x 21.2mm ID, Ea Part No.: AJ0-7846 - Holder Part No.: AJ0-8223