Amphetamines in Urine by LC/MS/MS on Gemini 5µm C18 150 x 3.0

LC Conditions

Column

Gemini 5 µm C18 110 Å, LC Column 150 x 3 mm, Ea

Brand

Gemini

Part No

00F-4435-Y0

Phase Name

C18

Elution Type

Isocratic

Mobile Phase

A: 10mM Ammonium formate w/0.1% formic acid

B: Acetonitrile

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

90

10

Flow Rate

0.60 mL/min

Temperature

22°C

Detection

Mass Spectrometer (MS) @ (450°C)

Detection Info

Sample Notes

SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, Gemini C18 4 x 2.0mm, 10/Pk Part No.: AJ0-7596 - Holder Part No.: KJ0-4282

Sample Preparation Details

Description

Strata-X-C 33 µm Polymeric Strong Cation, 30 mg / 1 mL, Tubes, 100/Pk

Part No

8B-S029-TAK

Pretreatment Note

to prevent the loss of amphetamine and methamphetamine during solvent evaporation, add two drops of conc. HCl before the evaporation step

Condition 1

1 mL Methanol

Condition 2

1 mL Water

Load

Load Sample

Wash 1

1 mL Water

Wash 2

1 mL 0.1M Acetic Acid

Wash 3

1 mL Methanol

Elution 1

1 mL 2% of ammonium hydroxide (28-30%) in methanol

Evaporation

Evaporate to dryness

Reconstitute

200 µL Mobile Phase