THC and Metabolite in Plasma by LC/MS/MS on Gemini C18 5µm 50 x 3.0

LC Conditions

Column

Gemini 3 µm C18 110 Å, LC Column 50 x 3 mm, Ea

Brand

Gemini

Part No

00B-4439-Y0

Phase Name

C18

Elution Type

Gradient

Mobile Phase

A: 10mM NH4HCO3 (pH 10)

B: Acetonitrile

Gradient Profile

Step No.

Time(min)

%A

%B

1

0.1

85

15

2

3

5

95

3

3.5

5

95

4

3.6

85

15

5

4.1

85

15

Flow Rate

0.80 mL/min

Temperature

22°C

Detection

Mass Spectrometer (MS) @ (450°C)

Detection Info

Sample Notes

SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, Gemini C18 4 x 2.0mm, 10/Pk Part No.: AJ0-7596 - Holder Part No.: KJ0-4282

Sample Preparation Details

Description

Strata-X-C 33 µm Polymeric Strong Cation, 30 mg / 1 mL, Tubes, 100/Pk

Part No

8B-S029-TAK

Condition 1

1 mL Methanol

Condition 2

1 mL DI Water

Load

Load 2.00 mL Sample diluted in 1mL Plasma diluted 1:1 with Water

Wash 1

1 mL 0.1N HAC

Wash 2

1 mL 30% ACN in 0.1N HAC

Wash 3

0.2

Elution 1

1 mL 2% HAC in CH3CN by gravity

Evaporation

Evaporate to dryness

Reconstitute

100 µL 15:85/ACN:10mM NH4HCO3, pH=10.0