Healthcare Opiates from Urine by Screen C on a ZB-Drug-1

GC Conditions

Column

Zebron ZB-Drug-1, GC Cap. Column 10 m x 0.18 mm x 0.18 µm, Ea

Part No

7CD-G023-08

Phase Name

ZB-Drug-1

Injection

Split 10:1 @ 260°C

Carrier Gas

Constant Flow Helium, 0.7 mL/min

Detection

Mass Selective (MSD) @ (320°C)

Detection Info

Sample Notes

Analytes are 300ng/mL in urine, extracted by SPE, and derivatized with 1.Hydroxylamine 2.BSTFA Sim ions: (Quant ion*) D6-Codeine (*377,349): Codeine (*371,343,234): D3-Morphine (*432,417): Morphine (*429,414,401): Hydrocodone (*386,297,371): D6-Hydromorphone (*361,450): Hydromorphone (*355,444,429): D6-Oxycodone (*480,465): Oxycodone (*474,459,460): Oxymorphone (*532,517,287)

Sample Preparation Details

Pretreatment Note

After extraction internal standards are added (morphine-d3, codeine-d6, hydromorphone-d6, oxymorphone-d6), then evaporated and reconstituted in 100µL of 50% hydroxylamine (reacted at 60ºC for 30 min), then evaporated, then reconstituted in BSTFA (reacted at 70ºC for 30 min) and injected

Condition 1

3 mL Methanol at 1 mL/min

Condition 2

3 mL Water at 1 mL/min

Condition 3

3 mL 100mM phosphate buffer (pH 6.0) at 1 mL/min

Load

Load 4.00 mL Sample diluted in Urine

Wash 1

3 mL Water at 1 mL/min

Wash 2

3 mL 100mM acetate buffer (pH 4.5) at 1 mL/min

Wash 3

3 mL Methanol at 1 mL/min

Dry

5 minutes >10 inches mercury

Elution 1

2 mL Methylene chloride: IPA: Ammonium hydroxide 76:20:4 at 1 mL/min

Evaporation

Evaporate to dryness

Reconstitute

50 µL Hydroxylamine