Biogenic Amines in Fish and Fish Products According to § 35 LMBG

LC Conditions

Column

Gemini 5 µm NX-C18 110 Å, LC Column 250 x 4.6 mm, Ea

Brand

Gemini

Part No

00G-4454-E0

Phase Name

NX-C18

Elution Type

Gradient

Mobile Phase

A: Water + 100 mM NaOAc, pH 4.5 + 10 mM Sodium 1-octanesulfonate

B: Water + 155 mM NaOAc, pH 4.5 + 10 mM Sodium 1-octanesulfonate / ACN (77:23)

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

80

20

2

20

60

40

3

28

25

75

4

34

25

75

5

34.1

0

100

6

50

0

100

7

50.1

80

20

8

60

80

20

Flow Rate

1.00 mL/min

Temperature

20°C

Detection

Fluorescence (FLUOR) @ (20°C)

Detection Info

Sample Notes

The application was kindly provided by the Amt für Verbraucherschutz in Mettmann, Germany.