Pharma ABN Test Mix on Gemini-NX

LC Conditions

Column

Synergi 4 µm Polar-RP 80 Å, LC Column 150 x 4.6 mm, Ea

Brand

Synergi

Part No

00F-4336-E0

Phase Name

Polar-RP

Elution Type

Gradient

Mobile Phase

A: Phosphate Buffer, pH 2.5

B: Acetonitrile

C: 0

D: 0

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

95

5

2

8

20

80

3

10

20

80

4

10.5

95

5

5

12

95

5

Flow Rate

1.00 mL/min

Temperature

22°C

Detection

UV-Vis Abs.-Diode Array (PDA) @ (22°C)

Detection Info

Sample Notes

SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, Polar-RP 4 x 3.0mm ID, 10/Pk Part No.: AJ0-6076 - Holder Part No.: KJ0-4282