LC/MS 30mer DNA Sequence (Figure 2)

LC Conditions

Column

Clarity 3 µm Oligo-RP, LC Column 150 x 2 mm, Ea

Brand

Clarity LC

Part No

00F-4441-B0

Phase Name

Oligo-RP

Elution Type

Gradient

Mobile Phase

A: 280mM HFIP/2.8mM TEA in 98:2 Water/MeOH

B: 280mM HFIP/2.8mM TEA in 98:2 MeOH/Water

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

95

5

2

5

90

10

3

15

70

30

Flow Rate

0.20 mL/min

Temperature

50°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (365°C)

Detection Info

Sample Notes

SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, Clarity Oligo-RP 4 x 2.0mm, 10/Pk Part No.: AJ0-8134 - Holder Part No.: KJ0-4282