Steroids in Standard Urine on Gemini 5µm C18 150 x 3mm ID

LC Conditions

Column

Gemini 5 µm C18 110 Å, LC Column 150 x 3 mm, Ea

Brand

Gemini

Part No

00F-4435-Y0

Phase Name

C18

Elution Type

Gradient

Mobile Phase

A: 0.1 % Formic Acid in Water

B: 0.1 % Formic Acid in 95% Acetonitrile/Water

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

60

40

2

4

100

0

3

15

25

75

4

15.1

40

60

5

20

40

60

Flow Rate

0.60 mL/min

Temperature

22°C

Detection

Mass Spectrometer (MS) @ (450°C)

Detection Info

Sample Notes

SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, Gemini C18 4 x 2.0mm, 10/Pk Part No.: AJ0-7596 - Holder Part No.: KJ0-4282

Sample Preparation Details

Description

Strata-X 33 µm Polymeric Reversed Phase, 30 mg / 1 mL, Tubes, 100/Pk

Part No

8B-S100-TAK

Condition 1

1 mL Methanol

Condition 2

1 mL Water

Load

Load 1.00 mL Sample diluted in Human serum or urine

Wash 1

1 mL Water

Wash 2

1 mL 40% Methanol in Water

Elution 1

1 mL Methanol

Evaporation

Evaporate to dryness

Reconstitute

100 µL 100 uL acetonitrile/water 40:60