Phencyclidine from urine by Screen C on a ZB-Drug1

GC Conditions

Column

Zebron ZB-Drug-1, GC Cap. Column 10 m x 0.18 mm x 0.18 µm, Ea

Part No

7CD-G023-08

Phase Name

ZB-Drug-1

Injection

Split 10:1 @ 260°C

Carrier Gas

Constant Flow Helium, 0.7 mL/min

Detection

Mass Selective (MSD) @ (320°C)

Detection Info

Sample Notes

Analytes 25ng/mL in urine Sim ions: (Quant ion*) D5-Phencyclidine (*205,248): Phencyclidine (*200,243,186)

Sample Preparation Details

Condition 1

3 mL Methanol at 1 mL/min

Condition 2

3 mL Water at 1 mL/min

Condition 3

1 mL 100mM Phosphate buffer pH 6.0 at 1 mL/min

Sample Pretreatment

6mL Barrel new part no.

Load

Load 2.00 mL Sample diluted in Urine

Wash 1

3 mL Water at 1 mL/min

Wash 2

1 mL 100mM acetic acid at 1 mL/min

Wash 3

3 mL Methanol at 1 mL/min

Dry

5 minutes at > 10 inch Hg

Elution 1

3 mL MeCl2NH3OH (78:20:2) at 1 mL/min

Evaporation

Evaporate to dryness

Reconstitute

50 µL Ethyl acetate