6-MAM from urine by Screen C on a ZB-Drug1

GC Conditions

Column

Zebron ZB-Drug-1, GC Cap. Column 10 m x 0.18 mm x 0.18 µm, Ea

Part No

7CD-G023-08

Phase Name

ZB-Drug-1

Injection

Split 10:1 @ 260°C

Carrier Gas

Constant Flow Helium, 0.7 mL/min

Detection

Mass Selective (MSD) @ (300°C)

Detection Info

Sample Notes

Analytes are 10ng/mL in urine derivatized with BSTFA Sim ions: (Quant ion*) D3-6-MAM (*402,403): 6-MAM (*399,400,340)

Sample Preparation Details

Condition 1

3 mL Methanol at 1 mL/min

Condition 2

3 mL Water at 1 mL/min

Condition 3

1 mL 100mM phosphate buffer (pH6.0) at 1 mL/min

Load

Load 4.00 mL Sample diluted in Urine

Wash 1

3 mL Water at 1 mL/min

Wash 2

2 mL 100mM acetate buffer (pH 4.5) at 1 mL/min

Wash 3

3 mL Methanol at 1 mL/min

Dry

10minutes at > 10 inch Hg

Elution 1

3 mL MeCl2NH3OH (78:20:2) at 1 mL/min

Evaporation

Evaporate to dryness

Reconstitute

50 µL BSTFA