Benzoylecgonine from urine by Screen C on a ZB-Drug1

GC Conditions

Column

Zebron ZB-Drug-1, GC Cap. Column 10 m x 0.18 mm x 0.18 µm, Ea

Part No

7CD-G023-08

Phase Name

ZB-Drug-1

Injection

Split 10:1 @ 260°C

Carrier Gas

Constant Flow Helium, 55.0 cm/sec

Detection

Mass Selective (MSD) @ (320°C)

Detection Info

Sample Notes

Analytes are 300ng/mL in urine derivatized with BSTFA Sim ions: (Quant ion*) D3-Benzoylecgonine (*243,259,364): Benzoylecgonine (*240,256,361)

Sample Preparation Details

Condition 1

3 mL Methanol at 1 mL/min

Condition 2

3 mL Water at 1 mL/min

Condition 3

1 mL 100mM phosphate buffer (pH 6.0) at 1 mL/min

Load

Load 2.00 mL Sample diluted in Urine

Wash 1

3 mL Water at 1 mL/min

Wash 2

2 mL 100mM HCl at 1 mL/min

Wash 3

3 mL Methanol at 1 mL/min

Dry

5 minutes at > 10 inch Hg

Elution 1

3 mL MeCl2NH3OH (78:20:2) at 1 mL/min

Evaporation

Evaporate to dryness

Reconstitute

50 µL BSTFA