Carboxy-THC from urine by Screen C on a ZB-Drug1

GC Conditions

Column

Zebron ZB-Drug-1, GC Cap. Column 10 m x 0.18 mm x 0.18 µm, Ea

Part No

7CD-G023-08

Phase Name

ZB-Drug-1

Injection

Split 10:1 @ 280°C

Carrier Gas

Constant Flow Helium, 55.0 cm/sec

Detection

Mass Selective (MSD) @ (320°C)

Detection Info

Sample Notes

Analytes are 15ng/mL in urine derivatized with BSTFA Sim ions: (Quant ion*) D3-Carboxy-thc (*374,476,477): Carboxy-thc (*371,473,474)

Sample Preparation Details

Pretreatment Note

After drying the cartridge (before elution step) add 200 µL hexane and aspirate to remove any residual moisture.

Condition 1

3 mL Methanol at 1 mL/min

Condition 2

3 mL Water at 1 mL/min

Condition 3

1 mL 100mM Acetate buffer (pH 3) at 1 mL/min

Load

Load 2.00 mL Sample diluted in Urine

Wash 1

2 mL Water at 1 mL/min

Wash 2

2 mL 100 mM HCI/acetonitrile (95:5) at 1 mL/min

Dry

5 minutes at > 10 inch Hg

Elution 1

3 mL Hexane/ethyl acetate (50:50) at 1 mL/min

Evaporation

Evaporate to dryness

Reconstitute

50 µL BSTFA