Expanded amphetamines from urine by Screen C on a ZB-Drug1

GC Conditions

Column

Zebron ZB-Drug-1, GC Cap. Column 10 m x 0.18 mm x 0.18 µm, Ea

Part No

7CD-G023-08

Phase Name

ZB-Drug-1

Injection

Split 10:1 @ 260°C

Carrier Gas

Constant Flow Helium, 0.5 mL/min

Detection

Mass Selective (MSD) @ (320°C)

Detection Info

Sample Notes

Analytes are 500ng/mL in urine derivatized with HFBA Sim ions: (Quant ion*) D11-Amphetamine (*244,128): Amphetamine (*240,118,117): D14-Methamphetamine (*261,213): Methamphetamine (*254,210,118): D5-MDA (*167,380,244): MDA (*162,375,240): D5-MDMA (*258,213,164): MDMA (*254,210,389): D5-MDEA (*273,241,408): MDEA (*268,240,403)

Sample Preparation Details

Condition 1

3 mL Methanol at 1 mL/min

Condition 2

3 mL Water at 1 mL/min

Condition 3

3 mL 100mM phosphate buffer (pH 6.0) at 1 mL/min

Load

Load 2.00 mL Sample diluted in Urine

Wash 1

3 mL Water at 1 mL/min

Wash 2

1 mL 100mM acetic acid at 1 mL/min

Wash 3

3 mL Methanol at 1 mL/min

Dry

5 minutes at 10 in Hg

Elution 1

3 mL MeCl2NH4OH (78:20:2) at 1 mL/min

Evaporation

Evaporate to dryness

Reconstitute

100 µL initial mobile phase