Barbiturates from urine by Screen C on a ZB-Drug1

GC Conditions

Column

Zebron ZB-Drug-1, GC Cap. Column 10 m x 0.18 mm x 0.18 µm, Ea

Part No

7CD-G023-08

Phase Name

ZB-Drug-1

Injection

Split 10:1 @ 280°C

Carrier Gas

Constant Flow Helium, 0.6 mL/min

Detection

Mass Selective (MSD) @ (320°C)

Detection Info

Sample Notes

Analytes are 300ng/mL in urine derivatized with TMPAH Sim ions: (Quant ion*) D5-Butalbital (*201,184,214): Butalbital (*196,181,138): Amobarbital (*169,184,225): Pentobarbital (*169,184,225): Secobarbital (*196,181,138): D5-Phenobarbital (*233,151,122): Phenobarbital (*232,146,117)

Sample Preparation Details

Condition 1

3 mL Methanol at 1 mL/min

Condition 2

3 mL Water at 1 mL/min

Condition 3

1 mL 100mM phosphate buffer (pH 5.0) at 1 mL/min

Load

Load 2.00 mL Sample diluted in Urine

Wash 1

3 mL Water at 1 mL/min

Wash 2

1 mL 100mM acetic acid at 1 mL/min

Dry

Dry column (5 minutes at > 10 inches Hg), add 2 mL of hexane and aspirate

Elution 1

3 mL Hexane/ethyl acetate (50:50) at 1 mL/min

Evaporation

Evaporate to dryness

Reconstitute

100 µL Acetonitrile/TMPAH