Ion Pairing & LC/MS Influence of Oligonucleotides (Figure 6c)

LC Conditions

Column

Clarity 3 µm Oligo-RP, LC Column 100 x 2 mm, Ea

Brand

Clarity LC

Part No

00D-4441-B0

Phase Name

Oligo-RP

Elution Type

Gradient

Mobile Phase

A: 200mM HFIP/8mMTEA/2% MeOH

B: 200mM HFIP/8mMTEA/98% MeOH

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

95

5

2

5

90

10

3

10

85

15

4

60

70

30

Flow Rate

0.25 mL/min

Temperature

50°C

Detection

Mass Spectrometer (MS) @ (365°C)

Detection Info

Sample Notes

SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, Clarity Oligo-RP 4 x 2.0mm, 10/Pk Part No.: AJ0-8134 - Holder Part No.: KJ0-4282