Interferon Alpha intact & deamidated on Jupiter 3u C18 and Jupiter 5u C4

LC Conditions

Column

Jupiter 3 µm C18 300 Å, LC Column 150 x 2 mm, Ea

Brand

Jupiter

Part No

00F-4263-B0

Phase Name

C18

Elution Type

Gradient

Mobile Phase

A: 0.1% TFA and 2% Acetonitrile in Water

B: 0.085% TFA, 90% Acetonitrile in Water

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

80

20

2

10

20

80

3

15

10

90

Flow Rate

0.30 mL/min

Temperature

25°C

Detection

UV-Vis Abs.-Diode Array (PDA) @ (25°C)

Detection Info

Sample Notes

SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, Widepore C18 4 x 2.0mm, 10/Pk Part No.: AJ0-4320 - Holder Part No.: KJ0-4282