Increase Sample Load by Increasing Column ID on Gemini-NX 5µm C18

LC Conditions

Column

Gemini 5 µm NX-C18 110 Å, LC Column 250 x 21.2 mm, AXIA Packed, Ea

Brand

Gemini

Part No

00G-4454-P0-AX

Phase Name

NX-C18

Elution Type

Gradient

Mobile Phase

A: Acetonitrile

B: 0.5% TFA in Water

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

5

95

2

25

95

5

Flow Rate

30.00 mL/min

Temperature

22°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info

Sample Notes

On-column injection of 96 mg. Compare with App ID 18077.