Poly dT 12-18 standard on Oligo-RP (modifying TEA/HFIP Ratio)

LC Conditions

Column

Clarity 3 µm Oligo-RP, LC Column 150 x 2 mm, Ea

Brand

Clarity LC

Part No

00F-4441-B0

Phase Name

Oligo-RP

Elution Type

Gradient

Mobile Phase

A: TEAA/HFIP/2%MeOH in Water

B: Acetonitrile

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

100

0

2

10

90

10

3

30

70

30

Flow Rate

0.20 mL/min

Temperature

22°C

Detection

Mass Spectrometer (MS) @ (22°C)

Detection Info

Sample Notes

Different ratios of HFIP to TEA were investigated using the poly dT 12-18 standard mixture. HFIP was fixed at 200 mM and different levels of TEA were used between 1 and 20 mM (4mm-red trace {pH 7.6}, 8mm-blue trace{pH8.0} and 14mm -black trace{pH8.3}). Optimal retention, MS response, and resolution were observed for the 200 mM HFIP./ 8mM TEA mobile phase mixture.