Melamine Extract from Milk by MSD on ZB-5ms
GC Conditions
Column
Zebron ZB-5MS, GC Cap. Column 30 m x 0.25 mm x 0.25 µm, Ea
Part No
7HG-G010-11
Phase Name
ZB-5MS
Injection
Splitless @ 280°C
Carrier Gas
Constant Flow Helium, 0.9 mL/min
Detection
Mass Selective (MSD) @ (320°C)
Detection Info
Sample Notes
Analytes were extracted from powdered milk and derivatized by BSTFA. Chromatograms are extracted ions of quantitation ions of Melamine (342) and Cyanuric Acid (345). Injection was pulsed splitless at 12 psi for 0.25 min.
Sample Preparation Details
Condition 1
3 mL Methanol at 1.0 mL/min
Condition 2
3 mL Water at 1.0 mL/min
Load
Load 1.00 mL Sample diluted in Acetonitrile:Water
Wash 1
1 mL Acetonitrile:Water (50/50); 2 aliquots of 500 uL at 1.0 mL/min
Wash 2
0.5 mL Methanol:Water (50/50) at 1.0 mL/min
Elution 1
1.5 mL 5% Ammonium Hydroxide in Methanol (2 aliquots of 750 uL) at 1.0 mL/min
Evaporation
Evaporate to dryness
Reconstitute
1 µL 90/10 : ACN/100mM ammonium formate, pH 3.2
Order Items Used in This Application
- 1. Melamine
- 2. Cyanuric acid
-OEChem-06211003152D<br><br> 15 15 0 0 0 0 0 0 0999 V2000<br> 4.2690 -1.3450 0.0000 N 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.4030 0.1550 0.0000 N 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.1350 0.1550 0.0000 N 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.5369 -1.3450 0.0000 N 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.0010 -1.3450 0.0000 N 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.2690 1.6550 0.0000 N 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.4030 -0.8450 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.1350 -0.8450 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.2690 0.6550 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.0000 -1.0350 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.5369 -1.9650 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.5380 -1.0350 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.0010 -1.9650 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.8059 1.9650 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.7320 1.9650 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 1 7 2 0 0 0 0<br> 1 8 1 0 0 0 0<br> 2 7 1 0 0 0 0<br> 2 9 2 0 0 0 0<br> 3 8 2 0 0 0 0<br> 3 9 1 0 0 0 0<br> 4 7 1 0 0 0 0<br> 4 10 1 0 0 0 0<br> 4 11 1 0 0 0 0<br> 5 8 1 0 0 0 0<br> 5 12 1 0 0 0 0<br> 5 13 1 0 0 0 0<br> 6 9 1 0 0 0 0<br> 6 14 1 0 0 0 0<br> 6 15 1 0 0 0 0<br>M END
Compound Name
Melamine
CID
7955
Molecular Formula
C3H6N6
Molecular Weight
126
No. Hydrogen Bond Acceptors
6
No. Hydrogen Bond Donors
3
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