Expanded healthcare opiates (propyl derivatives) from urine by Screen C on a ZB-Drug1

GC Conditions

Column

Zebron ZB-Drug-1, GC Cap. Column 10 m x 0.18 mm x 0.18 µm, Ea

Part No

7CD-G023-08

Phase Name

ZB-Drug-1

Injection

Split 10:1 @ 260°C

Carrier Gas

Constant Flow Helium, 0.7 mL/min

Detection

Mass Selective (MSD) @ (320°C)

Detection Info

Sample Notes

Analytes are 12 µg/mL derivatized with propionic anhydride (Tramadols are 24µg/mL) Sim ions: (Quant ion*) D4-Meperidine (*251,222): Meperidine (*247,218,246): Tramadol (*58,188): O-Desmethyltramadol (*58,174): D4-Normeperidine (*191,236): Normeperidine (*187,232,289): N-Desmethyltramadol (*305,276,248): Hydrocodone (*299,242,214): D6-Codeine (*361,288,235): Codeine (*355,282,229): D6-Oxycodone (*377,320): Oxycodone (*371,314,298): D6-Hydromorphone (*291,347): Hydromorphone (*285,341,228): D3-Morphine (*344,271,400): Morphine (*341,268,397): Oxymorphone (*357,300,413): Norcodeine (*223,397,101)

Sample Preparation Details

Condition 1

3 mL Methanol at 1 mL/min

Condition 2

3 mL Water at 1 mL/min

Condition 3

1 mL 100mM phosphate buffer (pH 6.0) at 1 mL/min

Load

Load 2.00 mL Sample diluted in Urine

Wash 1

3 mL Water at 1 mL/min

Wash 2

3 mL 100mM acetate buffer (pH 4.5) at 1 mL/min

Wash 3

3 mL Methanol at 1 mL/min

Dry

Dry column (5 minutes at > 10 inches Hg)

Elution 1

3 mL CH2Cl2/IPA/NH4OH (76:20:4) at 1 mL/min

Evaporation

Evaporate to dryness

Reconstitute

0 µL Pyridine/Propionic anhydride