Pharma ABN Test 12 Mix on Luna 5um C18(2) 250x4.6mm 1mL/min

LC Conditions

Column

Luna 5 µm C18(2) 100 Å, LC Column 250 x 4.6 mm, Ea

Brand

Luna

Part No

00G-4252-E0

Phase Name

C18(2)

Elution Type

Gradient

Mobile Phase

A: water w/ 0.1% formic acid

B: acetonitrile w/ 0.1% formic acid

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

95

5

2

2.79

95

5

3

36.15

5

95

4

36.38

95

5

5

50.93

95

5

Flow Rate

1.00 mL/min

Temperature

45°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (25°C)

Detection Info

Sample Notes

SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, C18 4 x 3.0mm, 10/Pk Part No.: AJ0-4287 - Holder Part No.: KJ0-4282