Simultaneous Extraction of Melamine and Cyanuric acid from Strata Melamine on Luna HILIC

LC Conditions

Column

Luna 3 µm HILIC 200 Å, LC Column 100 x 2 mm, Ea

Brand

Luna

Part No

00D-4449-B0

Phase Name

HILIC

Elution Type

Isocratic

Mobile Phase

A: 90/10 ACN/100 mM Ammonium Formate, pH 3.2

Gradient Profile

Step No.

Time(min)

%A

%B

1

3.5

100

0

Flow Rate

0.40 mL/min

Temperature

22°C

Detection

Mass Spectrometer (MS) @ (22°C)

Detection Info

Sample Notes

SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, HILIC 4 x 2.0mm, 10/Pk Part No.: AJ0-8328 - Holder Part No.: KJ0-4282

Sample Preparation Details

Condition 1

3 mL Methanol at 1.0 mL/min

Condition 2

3 mL Acetonitrile:Water (50/50) at 1.0 mL/min

Load

Load 3.50 mL Sample diluted in Acetonitrile:Water

Wash 1

0.5 mL Acetonitrile:Water (50/50); 2 aliquots of at 1.0 mL/min

Wash 2

0.5 mL Methanol:Water (50/50); 1 aliquot of at 1.0 mL/min

Wash 3

2 at 1.0

Dry

2 mins at 10 of Hg

Elution 1

0.5 mL Methanol at 1.0 mL/min

Elution 2

0.5 mL 5% Ammonium Hydroxide in Methanol; 2 aliquots of at 1.0 mL/min

Evaporation

Evaporate to dryness

Reconstitute

1 mL 90/10 : ACN/100mM ammonium formate, pH 3.2