Metanephrine and Normetanephrine on Luna HILIC 3um 50X2.0mm

LC Conditions

Column

Luna 3 µm HILIC 200 Å, LC Column 50 x 2 mm, Ea

Brand

Luna

Part No

00B-4449-B0

Phase Name

HILIC

Elution Type

Gradient

Mobile Phase

A: 95/5 ACN/100 mM ammonium formate, pH 3.2

B: 50/45/5 ACN/Water/100 mM ammonium formate, pH 3.2

Gradient Profile

Step No.

Time(min)

100

2.5

100

2.51

100

3.01

100

Flow Rate

0.40 mL/min

Temperature

22°C

Detection

Mass Spectrometer (MS) @ (22°C)

Detection Info

Sample Notes

LC/MS/MS & Chromatographic Condition MS Detector: API 3000 LC/MS/MS with ESI (TurboIonSpray®) (www.Appliedbiosystems.com), Positive Ion Mode; MRM; heater gas flow 7000 cc/min; heater temperature 550 °C; Column: LUNA 3µm HILIC, 50x2.0mm; Flow rate: 0.4ml/min ESI Source Conditions: NEB: 9.00 CUR: 10.00 IS: 3500.00 TEM: 550.00 CAD: 11.00 CE 23.00 Normetanephrine CE 25.00 Metanephrine Analyte Q1 Q3 Time DP FP CE CXP NMN 166.4 134.2 150 61 370 23 8 MN 180.4 148.2 150 71 370 25 8

Order Items Used in This Application

00B-4449-B0

Luna 3 µm HILIC 200 Å, LC Column 50 x 2 mm, Ea

View Product

1. Metanephrine
2. Normetanephrine

-OEChem-06211003162D<br><br> 29 29  0     1  0  0  0  0  0999 V2000<br>    3.7320    1.6550    0.0000 O   0  0  0  0  0  0  0  0  0  0  0  0<br>    2.8660   -1.8450    0.0000 O   0  0  0  0  0  0  0  0  0  0  0  0<br>    4.5981   -2.8450    0.0000 O   0  0  0  0  0  0  0  0  0  0  0  0<br>    5.4641    2.6550    0.0000 N   0  0  0  0  0  0  0  0  0  0  0  0<br>    4.5981    1.1550    0.0000 C   0  0  3  0  0  0  0  0  0  0  0  0<br>    4.5981    0.1550    0.0000 C   0  0  0  0  0  0  0  0  0  0  0  0<br>    5.4641    1.6550    0.0000 C   0  0  0  0  0  0  0  0  0  0  0  0<br>    3.7320   -0.3450    0.0000 C   0  0  0  0  0  0  0  0  0  0  0  0<br>    5.4641   -0.3450    0.0000 C   0  0  0  0  0  0  0  0  0  0  0  0<br>    3.7320   -1.3450    0.0000 C   0  0  0  0  0  0  0  0  0  0  0  0<br>    5.4641   -1.3450    0.0000 C   0  0  0  0  0  0  0  0  0  0  0  0<br>    4.5981   -1.8450    0.0000 C   0  0  0  0  0  0  0  0  0  0  0  0<br>    6.3301    3.1550    0.0000 C   0  0  0  0  0  0  0  0  0  0  0  0<br>    2.0000   -1.3450    0.0000 C   0  0  0  0  0  0  0  0  0  0  0  0<br>    4.5981    2.0050    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    5.6762    1.0724    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    6.0747    1.7627    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    3.1951   -0.0350    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    6.0010   -0.0350    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    4.9272    2.9650    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    6.0010   -1.6550    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    3.7320    2.2750    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    6.6401    2.6181    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    6.8671    3.4650    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    6.0201    3.6919    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    5.1350   -3.1550    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    2.3100   -0.8081    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    1.4631   -1.0350    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    1.6900   -1.8819    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>  1  5  1  0  0  0  0<br>  1 22  1  0  0  0  0<br>  2 10  1  0  0  0  0<br>  2 14  1  0  0  0  0<br>  3 12  1  0  0  0  0<br>  3 26  1  0  0  0  0<br>  4  7  1  0  0  0  0<br>  4 13  1  0  0  0  0<br>  4 20  1  0  0  0  0<br>  5  6  1  0  0  0  0<br>  5  7  1  0  0  0  0<br>  5 15  1  0  0  0  0<br>  6  8  2  0  0  0  0<br>  6  9  1  0  0  0  0<br>  7 16  1  0  0  0  0<br>  7 17  1  0  0  0  0<br>  8 10  1  0  0  0  0<br>  8 18  1  0  0  0  0<br>  9 11  2  0  0  0  0<br>  9 19  1  0  0  0  0<br> 10 12  2  0  0  0  0<br> 11 12  1  0  0  0  0<br> 11 21  1  0  0  0  0<br> 13 23  1  0  0  0  0<br> 13 24  1  0  0  0  0<br> 13 25  1  0  0  0  0<br> 14 27  1  0  0  0  0<br> 14 28  1  0  0  0  0<br> 14 29  1  0  0  0  0<br>M  END

Compound Name

Metanephrine

CID

21100

Molecular Formula

C10H15NO3

Molecular Weight

197

No. Hydrogen Bond Acceptors

No. Hydrogen Bond Donors

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