Opiates from urine by Strata-XC on a ZB-Drug1

GC Conditions

Column

Zebron ZB-Drug-1, GC Cap. Column 10 m x 0.18 mm x 0.18 µm, Ea

Part No

7CD-G023-08

Phase Name

ZB-Drug-1

Injection

Split 10:1 @ 260°C

Carrier Gas

Constant Flow Helium, 0.7 mL/min

Detection

Mass Selective (MSD) @ (320°C)

Detection Info

Sample Notes

Analytes are 300 ng/mL in urine derivatized with propionic anhydride Sim ions: (Quant ion*) D6-Codeine (*361,288,235): Codeine (*355,282,229): D3-Morphine (*344,271,400): Morphine (*341,268,397)

Sample Preparation Details

Condition 1

1 mL Methanol at 1 mL/min

Condition 2

1 mL Water at 1 mL/min

Load

Load 1.00 mL Sample diluted in Urine

Wash 1

1 mL 0.1 N HCl at 1 mL/min

Wash 2

1 mL Methanol at 1 mL/min

Dry

10 min @ >10 Hg

Elution 1

1 mL Methanol with 5% ammonium hydroxide at 1 mL/min

Evaporation

Evaporate to dryness

Reconstitute

50 µL Ethyl acetate