Benzoylecgonine from urine by Strata-XC on a ZB-Drug1

GC Conditions

Column

Zebron ZB-Drug-1, GC Cap. Column 10 m x 0.18 mm x 0.18 µm, Ea

Part No

7CD-G023-08

Phase Name

ZB-Drug-1

Injection

Split 10:1 @ 260°C

Carrier Gas

Constant Flow Helium, 55.0 cm/sec

Detection

Mass Selective (MSD) @ (320°C)

Detection Info

Sample Notes

Analytes are 300ng/mL extracted from urine then derivatized with BSTFA Sim ions: (Quant ion*) D3-Benzoylecgonine (*85,243,364): Benzoylecgonine (*82,240,361)

Sample Preparation Details

Condition 1

1 mL Methanol at 1 mL/min

Condition 2

1 mL Water at 1 mL/min

Condition 3

1 at 1

Load

Load 1.00 mL Sample diluted in Urine

Wash 1

1 mL HCl (0.1N) at 1 mL/min

Wash 2

1 mL Methanol at 1 mL/min

Wash 3

3 at 1

Dry

5 minutes at > 10 inch Hg

Elution 1

1 mL Methanol with 5% NH3OH at 1 mL/min

Evaporation

Evaporate to dryness

Reconstitute

50 µL BSTFA/Pyridine