Malachite Green and Related Dyes on Luna HST 2.5um C18(2) 50x2.0 Column

LC Conditions

Column

Luna 2.5 µm C18(2)-HST 100 Å, LC Column 50 x 2 mm, Ea

Brand

Luna

Part No

00B-4446-B0

Phase Name

C18(2)

Elution Type

Isocratic

Mobile Phase

A: 0.1% Formic acid in water

B: 0.1% Formic acid in 75:25 acetonitrile:methanol

Gradient Profile

Step No.

Time(min)

%A

%B

1

5

55

45

Flow Rate

0.40 mL/min

Temperature

0°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info