Methadone from urine by Strata-XC on a ZB-Drug1

GC Conditions

Column

Zebron ZB-Drug-1, GC Cap. Column 10 m x 0.18 mm x 0.18 µm, Ea

Part No

7CD-G023-08

Phase Name

ZB-Drug-1

Injection

Split 10:1 @ 280°C

Carrier Gas

Constant Flow Helium, 55.0 cm/sec

Detection

Mass Selective (MSD) @ (320°C)

Detection Info

Sample Notes

Methadone/Methadone-d9 is 300/600ng/mL respectively in urine Sim ions: (Quant ion*) D9-Methadone (*78,303): Methadone (*72,294,223)

Sample Preparation Details

Condition 1

1 mL Methanol at 1 mL/min

Condition 2

1 mL Water at 1 mL/min

Condition 3

2 at 1

Load

Load 1.00 mL Sample diluted in Urine

Wash 1

1 mL 0.1 N HCl at 1 mL/min

Wash 2

1 mL Methanol at 1 mL/min

Wash 3

3 at 1

Dry

5 minutes at > 10 inch Hg

Elution 1

5% Ammonium hydroxide in methanol

Evaporation

Evaporate to dryness

Reconstitute

50 µL Ethyl acetate