Analgesics using Kinetex 2.6µm C18 50 x 2.1mm ID (without KrudKatcher Ultra)

LC Conditions

Column

Kinetex 2.6 µm C18 100 Å, LC Column 50 x 2.1 mm, Ea

Brand

Kinetex

Part No

00B-4462-AN

Phase Name

C18

Elution Type

Gradient

Mobile Phase

A: Water with 0.1% Formic acid

B: Acetonitrile with 0.1% Formic Acid

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

90

10

2

2

10

90

3

2.5

10

90

4

2.51

90

10

Flow Rate

1.00 mL/min

Temperature

0°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info