EPA 8330 - High-Speed Screening of Explosives using Kinetex 2.6µm C18 100 x 4.6mm ID

LC Conditions

Official Method

EPA 8330

Column

Kinetex 2.6 µm C18 100 Å, LC Column 100 x 4.6 mm, Ea

Brand

Kinetex

Part No

00D-4462-E0

Phase Name

C18

Elution Type

Gradient

Mobile Phase

A: Water

B: Methanol

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

45

55

2

5

35

65

Flow Rate

1.40 mL/min

Temperature

0°C

Detection

UV-Vis Abs.-Diode Array (PDA) @ (22°C)

Detection Info

Sample Preparation Details

Description

Strata-XL 100 µm Polymeric Reversed Phase, 500 mg / 6 mL, Tubes, 30/Pk

Part No

8B-S043-HCH

Condition 1

10 mL Acetonitrile

Condition 2

30 mL DI Water

Load

Load Sample

Wash 1

Methanol/Water 5:95

Dry

3-5 min at 10 mm Hg

Elution 1

5 mL 85:15 Acetonitrile/Water

Evaporation

Evaporate to dryness

Reconstitute

0 µL initial mobile phase