Azo Dyes on Kinetex 2.6µm C18 150 x 4.6mm ID

LC Conditions

Column

Kinetex 2.6 µm C18 100 Å, LC Column 150 x 4.6 mm, Ea

Brand

Kinetex

Part No

00F-4462-E0

Phase Name

C18

Elution Type

Gradient

Mobile Phase

A: 0.1% phosphoric acid in water

B: 0.1% phosphoric acid in acetonitrile

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

75

25

2

15

5

95

3

17

5

95

4

17.01

75

25

5

20

75

25

Flow Rate

1.80 mL/min

Temperature

0°C

Detection

UV-Vis Abs.-Diode Array (PDA) @ (22°C)

Detection Info