EPA 531.1 Carbamate Pesticides at High Speed using Kinetex 2.6µm C18 50 x 2.1mm ID

LC Conditions

Official Method

EPA 531

Column

Kinetex 2.6 µm C18 100 Å, LC Column 50 x 2.1 mm, Ea

Brand

Kinetex

Part No

00B-4462-AN

Phase Name

C18

Elution Type

Gradient

Mobile Phase

A: Water with 0.1% H3PO4

B: Acetonitrile with 0.1% H3PO4

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

95

5

2

3

5

95

Flow Rate

1.00 mL/min

Temperature

0°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info

Sample Preparation Details

Description

Strata-X 33 µm Polymeric Reversed Phase, 30 mg / 3 mL, Tubes, 50/Pk

Part No

8B-S100-TBJ

Condition 1

1 mL Methanol

Condition 2

1 mL Loading buffer

Load

Load Sample

Wash 1

1 mL Loading buffer

Wash 2

1 mL Methanol/Water (15:85)

Dry

2 minutes at 10 mm Hg vacuum

Elution 1

0.5 mL 5% Formic acid in Methanol

Elution 2

0.5 mL 5% Formic acid in Methanol

Evaporation

Evaporate to dryness

Reconstitute

1 mL Mobile phase