Extraction of Tetracyclines using strata X-CW on Kinetex 2.6µm C18 50 x 2.1mm ID by LCMS
LC Conditions
Column
Kinetex 2.6 µm C18 100 Å, LC Column 50 x 2.1 mm, Ea
Brand
Kinetex
Part No
00B-4462-AN
Phase Name
C18
Elution Type
Gradient
Mobile Phase
A: 0.1% Formic acid in H2O
B: 0.1% Formic acid in ACN
Gradient Profile
Step No.
Time(min)
%A
%B
1
0
97
3
2
0.24
97
3
3
3.02
5
95
4
3.3
5
95
5
3.31
97
3
6
4.97
97
3
Flow Rate
450.00 mL/min
Temperature
0°C
Detection
Mass Spectrometer (MS) @ (22°C)
Detection Info
Sample Preparation Details
Description
Strata-X-CW 33 µm Polymeric Weak Cation, 30 mg / 3 mL, Tubes, 50/Pk
Part No
8B-S035-TBJ
Condition 1
1 mL Methanol at 2 mL/min
Condition 2
1 mL Ammonium acetate pH 5.5 at 2 mL/min
Load
Load 0.50 mL Sample diluted in 1 mL
Wash 1
1 mL Ammonium acetate pH 5.5 at 2 mL/min
Wash 2
1 mL Methanol at 1 mL/min
Dry
Dry for 2 mins at 10 of Hg
Elution 1
0.500 mL 2% FA/methanol at 1 mL/min
Elution 2
0.5 mL 2% FA/methanol at 1 mL/min
Evaporation
Evaporate to dryness
Reconstitute
200 µL Initial LC mobile phase (97/3)
Order Items Used in This Application
- 1. Oxytetracycline
- 2. Tetracycline
- 3. Chlorotetracycline
-OEChem-05081000432D<br><br> 57 60 0 1 0 0 0 0 0999 V2000<br> 6.9338 1.9246 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.0678 -1.5754 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 9.1837 1.8310 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.9338 -2.0754 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 8.6822 -2.1100 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.6839 -1.9818 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.4037 0.9488 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 10.5194 -2.1530 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.4075 -2.0995 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.1854 1.9592 0.0000 N 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.5357 -0.6029 0.0000 N 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.0678 0.4246 0.0000 C 0 0 2 0 0 0 0 0 0 0 0 0<br> 7.7998 0.4246 0.0000 C 0 0 2 0 0 0 0 0 0 0 0 0<br> 6.9338 0.9246 0.0000 C 0 0 2 0 0 0 0 0 0 0 0 0<br> 6.0678 -0.5754 0.0000 C 0 0 1 0 0 0 0 0 0 0 0 0<br> 5.1738 0.9593 0.0000 C 0 0 1 0 0 0 0 0 0 0 0 0<br> 8.6938 0.9593 0.0000 C 0 0 1 0 0 0 0 0 0 0 0 0<br> 7.7998 -0.5754 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.9338 -1.0754 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.1738 -1.1100 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 9.5998 0.4455 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.2678 0.4455 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 8.6938 -1.1100 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 8.1838 1.8195 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 9.5998 -0.5962 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.2678 -0.5962 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 10.5310 1.0024 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.3252 2.4692 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.0572 2.4492 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 10.5310 -1.1531 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.4037 -1.0995 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 11.4748 0.4671 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 11.4748 -0.6179 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.0743 1.2746 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.7933 1.2746 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.3969 1.2346 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.7149 1.2620 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 8.7171 2.1357 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.8676 2.3528 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.6505 1.5033 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.4707 2.2346 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.7578 -2.1123 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 8.8675 2.3644 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 10.5238 1.6223 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.6414 3.0025 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.7919 2.7854 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.0090 1.9359 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.3609 1.9087 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.5976 2.7530 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.7534 2.9897 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 9.2155 -2.4261 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 12.0105 0.7792 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 12.0105 -0.9299 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.0000 -0.9149 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.5334 0.0171 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 11.0528 -2.4692 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.8718 -2.4116 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 14 1 1 1 0 0 0<br> 1 41 1 0 0 0 0<br> 15 2 1 1 0 0 0<br> 2 42 1 0 0 0 0<br> 17 3 1 6 0 0 0<br> 3 43 1 0 0 0 0<br> 4 19 2 0 0 0 0<br> 5 23 1 0 0 0 0<br> 5 51 1 0 0 0 0<br> 6 20 2 0 0 0 0<br> 7 22 2 0 0 0 0<br> 8 30 1 0 0 0 0<br> 8 56 1 0 0 0 0<br> 9 31 1 0 0 0 0<br> 9 57 1 0 0 0 0<br> 16 10 1 1 0 0 0<br> 10 28 1 0 0 0 0<br> 10 29 1 0 0 0 0<br> 11 31 1 0 0 0 0<br> 11 54 1 0 0 0 0<br> 11 55 1 0 0 0 0<br> 12 14 1 0 0 0 0<br> 12 15 1 0 0 0 0<br> 12 16 1 0 0 0 0<br> 12 34 1 1 0 0 0<br> 13 14 1 0 0 0 0<br> 13 17 1 0 0 0 0<br> 13 18 1 0 0 0 0<br> 13 35 1 1 0 0 0<br> 14 36 1 0 0 0 0<br> 15 19 1 0 0 0 0<br> 15 20 1 0 0 0 0<br> 16 22 1 0 0 0 0<br> 16 37 1 0 0 0 0<br> 17 21 1 0 0 0 0<br> 17 24 1 0 0 0 0<br> 18 19 1 0 0 0 0<br> 18 23 2 0 0 0 0<br> 20 26 1 0 0 0 0<br> 21 25 2 0 0 0 0<br> 21 27 1 0 0 0 0<br> 22 26 1 0 0 0 0<br> 23 25 1 0 0 0 0<br> 24 38 1 0 0 0 0<br> 24 39 1 0 0 0 0<br> 24 40 1 0 0 0 0<br> 25 30 1 0 0 0 0<br> 26 31 2 0 0 0 0<br> 27 32 2 0 0 0 0<br> 27 44 1 0 0 0 0<br> 28 45 1 0 0 0 0<br> 28 46 1 0 0 0 0<br> 28 47 1 0 0 0 0<br> 29 48 1 0 0 0 0<br> 29 49 1 0 0 0 0<br> 29 50 1 0 0 0 0<br> 30 33 2 0 0 0 0<br> 32 33 1 0 0 0 0<br> 32 52 1 0 0 0 0<br> 33 53 1 0 0 0 0<br>M END
Compound Name
Oxytetracycline
CID
5280972
Molecular Formula
C22H24N2O9
Molecular Weight
460
No. Hydrogen Bond Acceptors
11
No. Hydrogen Bond Donors
7
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