Extraction of Sulfa drugs using Strata X-C on Kinetex 2.6µm C18 50 x 2.1mm ID by LCMS
LC Conditions
Column
Kinetex 2.6 µm C18 100 Å, LC Column 50 x 2.1 mm, Ea
Brand
Kinetex
Part No
00B-4462-AN
Phase Name
C18
Elution Type
Gradient
Mobile Phase
A: 0.1% Formic acid in H2O
B: 0.1% Formic acid in ACN
Gradient Profile
Step No.
Time(min)
%A
%B
1
0
95
5
2
0.24
95
5
3
3.02
5
95
4
3.3
5
95
5
3.31
95
5
6
4.97
95
5
Flow Rate
450.00 mL/min
Temperature
0°C
Detection
Mass Spectrometer (MS) @ (22°C)
Detection Info
Sample Preparation Details
Description
Strata-X-C 33 µm Polymeric Strong Cation, 30 mg / 3 mL, Tubes, 50/Pk
Part No
8B-S029-TBJ
Condition 1
1 mL Methanol at 2 mL/min
Condition 2
1 mL Water at 2 mL/min
Load
Load 0.50 mL Sample diluted in 0.5
Wash 1
1 mL 0.1M acetic acid at 2 mL/min
Wash 2
1 mL 25% methanol/0.1M acetic acid at 2 mL/min
Wash 3
1 at 2
Dry
2 min at 10Hg
Elution 1
0.5 mL 5% ammonium hydroxide/methanol at 1 mL/min
Elution 2
0.5 mL 5% ammonium hydroxide/methanol at 1 mL/min
Evaporation
Evaporate to dryness
Reconstitute
200 µL Initial LC mobile phase (95/5)
Order Items Used in This Application
- 1. Sulfadiazine
- 2. Sulfathiazole
- 3. Sulfamerazine
- 4. Sulfamethoxazole
- 5. Sulfaquinoxaline
-OEChem-06211003152D<br><br> 27 28 0 0 0 0 0 0 0999 V2000<br> 3.0000 0.6550 0.0000 S 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.0000 0.6550 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.0000 0.6550 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.0000 1.6550 0.0000 N 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.0000 -3.3450 0.0000 N 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.8660 3.1550 0.0000 N 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.7320 1.6550 0.0000 N 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.0000 -0.3450 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.1340 -0.8450 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.8660 -0.8450 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.0000 -2.3450 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.1340 -1.8450 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.8660 -1.8450 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.8660 2.1550 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.7320 3.6550 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.5981 2.1550 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.5981 3.1550 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 1.5970 -0.5350 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.4030 -0.5350 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.4631 1.9650 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 1.5970 -2.1550 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.4030 -2.1550 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.4631 -3.6550 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.5369 -3.6550 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.7320 4.2750 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.1350 1.8450 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.1350 3.4650 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 1 2 2 0 0 0 0<br> 1 3 2 0 0 0 0<br> 1 4 1 0 0 0 0<br> 1 8 1 0 0 0 0<br> 4 14 1 0 0 0 0<br> 4 20 1 0 0 0 0<br> 5 11 1 0 0 0 0<br> 5 23 1 0 0 0 0<br> 5 24 1 0 0 0 0<br> 6 14 1 0 0 0 0<br> 6 15 2 0 0 0 0<br> 7 14 2 0 0 0 0<br> 7 16 1 0 0 0 0<br> 8 9 2 0 0 0 0<br> 8 10 1 0 0 0 0<br> 9 12 1 0 0 0 0<br> 9 18 1 0 0 0 0<br> 10 13 2 0 0 0 0<br> 10 19 1 0 0 0 0<br> 11 12 2 0 0 0 0<br> 11 13 1 0 0 0 0<br> 12 21 1 0 0 0 0<br> 13 22 1 0 0 0 0<br> 15 17 1 0 0 0 0<br> 15 25 1 0 0 0 0<br> 16 17 2 0 0 0 0<br> 16 26 1 0 0 0 0<br> 17 27 1 0 0 0 0<br>M END
Compound Name
Sulfadiazine
CID
5215
Molecular Formula
C10H10N4O2S
Molecular Weight
250
No. Hydrogen Bond Acceptors
6
No. Hydrogen Bond Donors
2
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