Selectivity Comparison for Steroids on Kinetex 2.6µm C18 150 x 4.6mm ID

LC Conditions

Column

Kinetex 2.6 µm C18 100 Å, LC Column 150 x 4.6 mm, Ea

Brand

Kinetex

Part No

00F-4462-E0

Phase Name

C18

Elution Type

Gradient

Mobile Phase

A: Water

B: ACN

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

75

25

2

12

35

65

3

12.1

75

25

4

16

75

25

Flow Rate

1.20 mL/min

Temperature

0°C

Detection

UV-Vis Abs.-Diode Array (PDA) @ (30°C)

Detection Info