Healthcare Opiates on Kinetex 2.6µm C18 50 x 2.1 mm ID by LC/MS

LC Conditions

Column

Kinetex 2.6 µm C18 100 Å, LC Column 50 x 2.1 mm, Ea

Brand

Kinetex

Part No

00B-4462-AN

Phase Name

C18

Elution Type

Gradient

Mobile Phase

A: 0.1% Formic acid and 5 mM ammonium acetate in H2O

B: 0.1% Formic acid and 5 mM ammonium acetate in 50-50ACN-Methanol

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

95

5

2

0.24

95

5

3

3.36

20

80

4

3.37

5

95

5

3.68

5

95

6

3.69

95

5

7

5.55

95

5

Flow Rate

400.00 mL/min

Temperature

0°C

Detection

Mass Spectrometer (MS) @ (22°C)

Detection Info