Peptide Map of unreduced Human Ig-G2 on Kinetex 2.6µm C18 150 x 2.1mm ID

LC Conditions

Column

Kinetex 2.6 µm C18 100 Å, LC Column 150 x 2.1 mm, Ea

Brand

Kinetex

Part No

00F-4462-AN

Phase Name

C18

Elution Type

Gradient

Mobile Phase

A: 0.1% TFA/2% ACN/Water

B: 0.09% TFA/98% ACN/Water

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

98

2

2

2

98

2

3

37

52

48

Flow Rate

1.00 mL/min

Temperature

0°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info