Water-soluble vitamins on Kinetex 2.6um HILIC 150 x 4.6mm

LC Conditions

Column

Kinetex 2.6 µm HILIC 100 Å, LC Column 150 x 4.6 mm, Ea

Brand

Kinetex

Part No

00F-4461-E0

Phase Name

HILIC

Elution Type

Gradient

Mobile Phase

A: Acetonitrile

B: 100mM Ammonium formate pH 3.2

C: Water

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

90

10

2

5

90

10

3

20

50

10

Flow Rate

1.00 mL/min

Temperature

0°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info