Extraction of Benzodiazepines Using Strata Screen-C on Kinetex 2.6µm C18 50 x 2.1mm ID by LCMS

LC Conditions

Column

Kinetex 2.6 µm C18 100 Å, LC Column 50 x 2.1 mm, Ea

Brand

Kinetex

Part No

00B-4462-AN

Phase Name

C18

Elution Type

Gradient

Mobile Phase

A: 0.1%FA in H2O

B: 0.1%FA in Acetonitrile

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

65

35

2

2

65

35

3

2.01

5

95

4

2.3

5

95

5

2.31

65

35

6

4.1

65

35

Flow Rate

450.00 mL/min

Temperature

0°C

Detection

Mass Spectrometer (MS) @ (22°C)

Detection Info

Sample Preparation Details

Description

Strata Screen-C (55 µm, 70 Å), 100 mg / 3 mL, Tubes, 50/Pk

Part No

8B-S016-EBJ

Condition 1

3 mL Methanol at 1 mL/min

Condition 2

3 mL Water at 1 mL/min

Condition 3

1 mL 100 mM phosphate buffer, pH 6 (adjust with phosphoric acid) at 1 mL/min

Load

Load 2.00 mL Sample diluted in 100 mm acetate buffer

Wash 1

2 mL Water at 1 mL/min

Wash 2

2 mL 20% acetonitrile/100 mm phosphate buffer, pH 6 at 1 mL/min

Dry

2-3 mins at 10 of Hg

Elution 1

2 mL Ethylacetate at 1 mL/min

Elution 2

2 mL Ethylacetate at 1 mL/min

Evaporation

Evaporate to dryness

Reconstitute

1 mL Initial mob phase(A/B=65/35)