Clarity OTX- MS Sensitivity of recovered 21mer at 500ng/mL from plasma

LC Conditions

Column

Clarity 3 µm Oligo-RP, LC Column 50 x 2 mm, Ea

Brand

Clarity LC

Part No

00B-4441-B0

Phase Name

Oligo-RP

Elution Type

Gradient

Mobile Phase

A: 1%HFIPA/ 0.1%DIEA/ water

B: 90% MeOH/ 0.075%HFIPA/ 0.0375% DIEA

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

90

10

2

20

65

35

Flow Rate

0.25 mL/min

Temperature

60°C

Detection

Mass Spectrometer (MS) @ (22°C)

Detection Info

Sample Notes

SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, Clarity Oligo-RP 4 x 2.0mm ID, 10/Pk Part No.: AJ0-8134 - Holder Part No.: KJ0-4282

Sample Preparation Details

Description

Clarity OTX 100 mg Plate, 100 mg/well 96-well plate, Ea

Part No

8E-S103-EGA

Condition 1

0.5 mL MeOH at 1 mL/min

Condition 2

0.5 mL Clarity OTX Equilibration Buffer at 1 mL/min

Load

Load 500.00 µL Sample diluted in OTX loading buffer/sample

Wash 1

0.5 mL Clarity OTX Wash Buffer at 1 mL/min

Wash 2

0.5 mL Clarity OTX Equilibration Buffer at 1 mL/min

Dry

10 um vaccuum

Elution 1

0.5 mL Clarity OTX Elution Buffer at 1 mL/min

Evaporation

Evaporate to dryness

Reconstitute

200 µL Mobile phase A