Verapamil Hydrochloride Related Substance HPLC Analysis (HP-EUR) on Luna 5u C8(2) 150 x 4.6mm ID

LC Conditions

Column

Luna 5 µm C8(2) 100 Å, LC Column 150 x 4.6 mm, Ea

Brand

Luna

Part No

00F-4249-E0

Phase Name

C8(2)

Elution Type

Gradient

Mobile Phase

A: 6.97g/l K2HPO4 buffer pH=7.2

B: Acetonitrile

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

63

37

2

22

63

37

3

27

35

65

4

35

35

65

5

36

63

37

6

50

63

37

Flow Rate

2.00 mL/min

Temperature

25°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info

Sample Notes

SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, C8 4 x 3.0mm ID, 10/Pk Part No.: AJ0-4290 - Holder Part No.: KJ0-4282