Propranolol on Lux 5 µm Cellulose-1 by Chiral SFC with EtOH dissolution solvent

LC Conditions

Column

Lux 5 µm Cellulose-1, LC Column 250 x 4.6 mm, Ea

Brand

Lux

Part No

00G-4459-E0

Phase Name

Cellulose-1

Elution Type

Isocratic

Mobile Phase

A: Methanol with 0.1% DEA

B: Carbon Dioxide

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

25

75

Flow Rate

2.50 mL/min

Temperature

35°C

Detection

Laser Polarimeter @ (22°C)

Detection Info

Sample Notes

Application using combination of UV-Vis detector (upper trace) and PDR-Chiral Advanced Laser Polarimeter (lower trace) courtesy of PDR-Chiral, Inc. 3mg in 100µL on-column injection of Propanolol. Sample was dissolved in Ethanol.