Mix on Synergi Polar-RP 5 µm by SFC

LC Conditions

Column

Synergi 4 µm Polar-RP 80 Å, LC Column 250 x 4.6 mm, Ea

Brand

Synergi

Part No

00G-4336-E0

Phase Name

Polar-RP

Elution Type

Gradient

Mobile Phase

A: Methanol

B: Carbon Dioxide

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

5

95

2

5.5

50

50

3

7.5

50

50

Flow Rate

3.00 mL/min

Temperature

40°C

Detection

UV-Vis Abs.-Fixed Wave. (UV) @ (40°C)

Detection Info

Sample Notes

SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, Silica 4 x 3.0mm, 10/Pk Part No.: AJ0-4348 - Holder Part No.: KJ0-4282