Metaprolol on Luna HILIC 5 µm by SFC

LC Conditions

Column

Luna 5 µm HILIC 200 Å, LC Column 250 x 4.6 mm, Ea

Brand

Luna

Part No

00G-4450-E0

Phase Name

HILIC

Elution Type

Gradient

Mobile Phase

A: Methanol with 0.1% DEA

B: Carbon Dioxide

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

10

90

2

2

10

90

3

12

40

60

4

14

40

60

5

15

10

90

Flow Rate

3.00 mL/min

Temperature

35°C

Detection

UV-Vis Abs.-Fixed Wave. (UV) @ (35°C)

Detection Info

Sample Notes

SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, Silica 4 x 3.0mm, 10/Pk Part No.: AJ0-4348 - Holder Part No.: KJ0-4282