LC/MS for Trypsin Digested Myoglobin-peptide mapping on Kinetex 2.6 µm C18 150 x 2.1mm

LC Conditions

Column

Kinetex 2.6 µm C18 100 Å, LC Column 150 x 2.1 mm, Ea

Brand

Kinetex

Part No

00F-4462-AN

Phase Name

C18

Elution Type

Gradient

Mobile Phase

A: 0.1% Formic Acid in water

B: 0.1% Formic Acid in Acetonitrile

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

97

3

2

20

35

65

3

22

5

95

4

22.1

97

3

Flow Rate

0.22 mL/min

Temperature

0°C

Detection

Mass Spectrometer (MS) @ (22°C)

Detection Info