LC/MS for Trypsin Digested BSA-peptide mapping on Kinetex 2.6 µm C18 150 x 4.6mm

LC Conditions

Column

Kinetex 2.6 µm C18 100 Å, LC Column 150 x 4.6 mm, Ea

Brand

Kinetex

Part No

00F-4462-E0

Phase Name

C18

Elution Type

Gradient

Mobile Phase

A: 0.1% Formic Acid in water

B: 0.1% Formic Acid in Acetonitrile

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

97

3

2

40

66.5

33.5

3

50

40

60

4

52

5

95

5

52.1

97

3

Flow Rate

1.20 mL/min

Temperature

0°C

Detection

Mass Spectrometer (MS) @ (22°C)

Detection Info