LC/MS for Trypsin Digested Myoglobin on Kinetex 2.6 µ C18, Halo 2.7µ C18, XBridge 3.5µ C18 phases

LC Conditions

Column

Kinetex 2.6 µm C18 100 Å, LC Column 150 x 4.6 mm, Ea

Brand

Kinetex

Part No

00F-4462-E0

Phase Name

C18

Elution Type

Gradient

Mobile Phase

A: 0.1% Formic Acid in water

B: 0.1% Formic Acid in Acetonitrile

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

97

3

2

25

32

68

3

27

5

95

4

27.1

97

3

Flow Rate

1.20 mL/min

Temperature

0°C

Detection

Mass Spectrometer (MS) @ (22°C)

Detection Info